Aloeverose is a new type of functional rare sugar, with a sweetness of about 70% of sucrose, a pure taste, almost no calories, and a number of health and medicinal functions such as hypoglycemia, antioxidant, and neuroprotection. In addition, Alozone Sugar is also one of the few sugar substitutes that can produce a Meladic reaction, so it can be used in some foods that require a high temperature reaction.
Currently, the industrial production of allulose is based on whole-cell enzyme-catalyzed method, i.e., the interconversion between fructose and allulose is achieved by sugar isomerase. However, the whole reaction process needs to be carried out under high temperature and high osmotic pressure conditions, which is a great challenge to the enzyme activity as well as the stability of its expression host.
In addition, fructose, as one of the dominant carbon sources that can be utilized by the host, is easily metabolized by the host bacteria without participating in the synthesis of alloxydose, which reduces the conversion rate of alloxydose. In short, the current difficulties in the production of domestic alloxan lie in the three major aspects of isomerase activity, reuse frequency and conversion rate.
Aiming at these industrialization difficulties, the scientific research team of Ecotron Biologicals used E. coli as a chassis, and through directed evolution and modification of the strain, fructose could not enter into the inside of E. coli cells during the whole-cell catalytic process, in order to reduce the non-purposeful depletion of fructose and to enhance the utilization efficiency of the substrate.
The E. coli strain was also domesticated to tolerate high temperatures and high fructose concentrations in order to improve the stability of the E. coli host in the catalytic environment and thus increase the number of reuse of the strain.
Finally, the enzyme anchoring technology was used to anchor the alloxan isomerase to the surface of E. coli, allowing the alloxan isomerase to grow on the surface of E. coli and catalyze the conversion of fructose into alloxan in the external environment, with the final performance increased up to 8-fold in comparison to the traditional method (intracellular catalysis).
The approved patent technology greatly accelerates the progress of the company's industrialized production of Alozone Sugar, which is a step forward for Strike Bio to create a green solution for health food with innovative bio-fermentation technology.
At present, in addition to the supply of high-quality raw materials, Strike Bio also supports professional scientific compounding and provides three-dimensional platform services such as upstream R&D and commercialization landing to meet the customized needs of different customers.
The company has cooperated with leading food and beverage companies as well as universities and colleges such as Jiangnan University to promote joint research and development projects on food innovations such as antimicrobial peptides, breast milk oligosaccharides, and acidulants (citric acid and malic acid).
As a synthetic biology platform company, Strontium Bio will work together with global partners to build a sustainable food future, and continue to develop healthy product solutions that meet people's nutritional needs.
Technology to the new, all things grow.